Skip to main content
. Author manuscript; available in PMC: 2011 Nov 4.
Published in final edited form as: Neuron. 2010 Nov 4;68(3):500–511. doi: 10.1016/j.neuron.2010.09.026

Fig. 2. D3R-PKC pathway is specific for T-type channels localized to the AIS.

Fig. 2

(A) 2-photon z-stack of cartwheel cell. Arrowheads: sites of Ca2+ transients detailed in (B). Left in AIS, right in dendrite.

(B) Voltage steps from −100 to −60 mV evoked a whole cell T-current (IT) and Ca2+ transients in the AIS and dendrite. Black: baseline, grey: in PMA.

(C-D) IT (C) and normalized ΔG/R (D) following activation of D3R-PKC pathway or block of T-type channels. Control currents were calculated as the relative IT over a time course similar to that allowed for drug wash-in (12 min). Ni2+ was iontophoresed locally to the AIS. All other drugs were added to the recording solution. Dots are single cells. Lines connecting dots in (D) link recordings made in the same cell in the AIS and dendrite. Dendritic recordings are denoted with a “D”. Bars are SEM. Asterisk: p < 0.05.