Table 1.
Characteristics of bacterial strains, plasmids, and phages used in the study
| Strains and plasmids | Relevant characteristics | Source or ref. |
|---|---|---|
| PRS1-Km | Extrachromosomal replicative form of the RS1 element carrying an intergenic KmR marker | 5 |
| RV-508 | V. cholerae O1 classical biotype strain that constitutively expresses CT, TCP, and other toxR-regulated gene products | 1 |
| 55V71 | Nontoxigenic (CTX-negative) environmental V. cholerae non-O1 non-O139 strain | This study |
| 55V71 (pRS1-Km) | Strain 55V71 carrying pRS1-Km | This study |
| KSF-1Φ | A filamentous phage isolated from strain 55V71 | This study |
| PKSF-1.1 | Replicative form of KSF-1Φ genome cloned into the XbaI site of pUC18 | This study |
| S-224 | Toxigenic V. cholerae O1 classical biotype strain | Collection |
| AF-1471, AK-31047 | Toxigenic V. cholerae O1 El Tor strains | Collection |
| AL-11089 | Toxigenic V. cholerae O139 strain | 5 |
| Env-99 | Environmental CTXΦ-negative V. cholerae O139 strain | 15 |
| Env-99 (RS1-Km) | Strain Env-99 lysogenized with RS1-KmΦ | This study |
| Env-99 (RS1-Km, pKSF1.1) | Env-99 (RS1-Km) electroporated with pKSF1.1 | This study |
| Env-002 | Environmental CTXΦ-negative V. cholerae O1 strain | 15 |
| Env-002 (RS1-Km) | Strain Env-002 lysogenized with RS1-KmΦ | This study |
| Env-002 (RS1-Km, pKSF-1.1) | Env-002 (RS1-Km) electroporated with pKSF1.1 | This study |
| SA-317, SA-406 | Clinical nontoxigenic V. cholerae O1 El Tor strains | 9 |
| SA-317 (RS1-Km) | Strain SA-317 lysogenized with RS1-KmΦ | This study |
| SA-406 (RS1-Km) | Strain SA-406 lysogenized with RS1-KmΦ | This study |
| AM-15714, AM-15729 | Clinical CTX-negative non-O1-non-0139 strains | Collection |
| AM-15714 (pRS1-Km) | Strain AM-15714 carrying pRS1-Km | This study |
| AM-15714 (pRS1-Km, pKSF1.1) | AM-15714(pRS1-Km) electroporated with pKSF1.1 | This study |
| AM-15729 (RS1-Km) | Strain AM-15729 lysogenized with RS1-KmΦ | This study |
| AM-15729 (RS1-Km, pKSF1.1) | AM-15729(RS1-Km) electroporated with pKSF1.1 | This study |