FIG. 2.
GFP immunostaining of serial brain sections 4 weeks after injecting AAV vectors into the hippocampus demonstrating widespread expression with AAV1, AAV5, and AAV8 relative to AAV2. (A) AAV1 GFP. (B) AAV2 GFP. (C) AAV5 GFP. (D) AAV8 GFP. Each vector was injected at a dose of 7 × 109 vector genomes. Every sixth section was processed over a 2.8-mm front-to-back extent of the brain. The sequence for the 15 sections shown in A–D is indicated to the left of the sections in A. Bar: 6.2 mm. A–D same magnification.