TABLE 1.
| (A) Tyrosine hydroxylase neurons after gene transfera | ||||
|---|---|---|---|---|
| Treatment | Dose | TH neurons | N | Comparison |
| Uninjected | — | 9458 ± 169 | 5 | |
| AAV8 Empty | 8 | 9502 ± 189 | 3 | Uninjected, NS |
| AAV8 GFP | 4 | 9471 ± 161 | 3 | |
| AAV8 GFP | 8 | 8031 ± 569 | 3 | Uninjected, P < 0.05 |
| AAV8 GFP | 10 | 6586 ± 829 | 5 | |
| AAV8 GFP | 50 | 5550 ± 516 | 3 | AAV8 GFP dose 4, P < 0.01 |
| AAV8 tau | 4 | 2112 ± 512 | 3 | AAV8 GFP equal dose, P < 0.001 |
| AAV8 tau | 10 | 2415 ± 637 | 3 | AAV8 GFP equal dose, P < 0.001 |
| AAV2 tau | 4 | 6526 ± 615 | 4 | AAV8 tau dose 4, P < 0.001 |
| AAV5 tau | 6 | 7491 ± 316 | 3 | AAV8 tau dose 4, P < 0.001 |
| (B) Rotational behavior after gene transferb | |||||
|---|---|---|---|---|---|
| Vector | Dose | Ipsilateral | Contralateral | N | P |
| Uninjected | — | 12.0 ± 5.5 | 6.7 ± 4.2 | 6 | NS |
| AAV8 GFP | 4 | 19.7 ± 5.2 | 12.3 ± 3.2 | 3 | NS |
| AAV8 GFP | 10 | 18.8 ± 6.6 | 7.6 ± 2.4 | 5 | NS |
| AAV5 tau | 6 | 6.7 ± 4.8 | 13.0 ± 12.5 | 3 | NS |
| AAV8 tau | 4 | 38.7 ± 8.1 | 2.7 ± 2.7 | 3 | <0.02 |
| AAV8 tau | 10 | 22.0 ± 3.2 | 3.0 ± 2.5 | 3 | <0.001 |
a
Number of substantia nigra pars compacta dopamine neurons (±SEM) estimated by stereology at the indicated dose (×109 vector genomes) 4 weeks after gene transfer (for the vector groups). Significant dose–response relationship for number of TH neurons found with the four doses of the AAV8 GFP vector by ANOVA, P < 0.02 (see Results). Other comparisons used Dunnet’s and Bonferroni’s multiple comparison tests (see Results).
b
Amphetamine-stimulated rotations (±SEM) 4 weeks after gene transfer (for the vector groups, dose × 109 vector genomes). Side-to-side differences were observed in the AAV8 tau but not the GFP or AAV5 tau vector groups (t test).