Figure 5.
Subcellular location of LAG-3 and CD4 in activated T cells. Activated CD4+ T cells were harvested, treated with pronase, fixed, permeabilized, stained with anti-LAG-3 mAb or anti-CD4 mAb plus either (A) anti-murine γ-tubulin or (B) anti-EEA1 and analyzed by confocal microscopy. Images are representative of three independent experiments. (C and D) Pearson coefficient was calculated using SlideBook software and more than 25 randomly selected cells of each group was analyzed for quantification of colocalization. Student’s t test was used for statistical analysis (***p<0.0001).