Figure 3.
Transcriptional inhibition of HBV gene expression after repetitive injection of polyI/C. Groups of sex-, age-, and HBeAg-matched mice were injected daily with saline (NaCl) or polyI/C for 3 days. At 24 h after the last injection, total RNA and transcriptionally competent nuclei were isolated from each liver. Results from three saline- and three polyI/C-injected mice are shown. (A) Northern blot of HBV and GAPDH steady-state RNA levels. (B) Nuclear run-on transcriptional analysis in nuclei isolated from the same mice. Nuclei of the three mice in each group were pooled. (C) Steady-state RNA levels of the indicated genes analyzed by reprobing the Northern blot from A with cDNA probes. (D) Steady-state RNA levels of the indicated genes analyzed by RPA.