Table 1.
Sequence similarities of regulatory elements encoded on pE88 to the characterized regulatory proteins UviA of C. perfringens, TxeR of C. difficile, and BotR/A of C. botulinum
| ORF name and size* | Annotation | UviA (185 aa) | TxeR (184 aa) | BotR/A (178 aa) |
|---|---|---|---|---|
| CTP5 (172 aa) | Frameshift, UviA homolog | 32% (48%) >185 aa | 31% (49%) >170 aa | 26% (43%) >178 aa |
| CTP10 (179 aa) | Putative σ-factor/DNA-binding protein | 24% (43%) >184 aa | 28% (47%) >82 aa | n.s. |
| CTP11 (184 aa) | Putative σ-factor/DNA-binding protein | 25% (44%) >180 aa | 24% (44%) >179 aa | n.s. |
| TetR (177 aa) | Positive regulator of tetX | 27% (49%) >183 aa | 26% (47%) >184 aa | 67% (85%) >177 aa |
Percentages indicate identical (in parentheses: similar) amino acids. n.s., not significant.
*
Not listed here is the two-component system CTP21/CTP22 and CTP4 (71 aa), which shows homology only to UviB (64 aa) of C. perfringens (40% identity over 67 aa).