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. 2003 Jan 21;100(3):1322–1327. doi: 10.1073/pnas.0237126100

Figure 5.

Figure 5

Activation of mGlu5 receptor potentiates the effect of adenosine A2A receptors on DARPP-32 phosphorylation at Thr-34. Neostriatal slices were pretreated with adenosine deaminase (10 μg/ml) for 60 min. Neostriatal slices were incubated with CGS21680 alone for 5 min (●), CGS21680 plus DHPG (100 μM) for 5 min (○), or CGS21680 plus MPEP (10 μM) (▴) in the continued presence of adenosine deaminase. In experiments with CGS21680 plus MPEP, neostriatal slices were incubated for a total of 15 min in the presence of MPEP (10 μM). MPEP was added at 0 min, and CGS21680 was added at 10 min of incubation. Data represent means ± SEM for five to seven experiments.