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(a) A digitally subtracted lensfree hologram of three moving sperms is generated from two successive frames (500 ms apart) (b) A microscopic image, that is digitally reconstructed from the lensfree differential hologram shown in (a), illustrates the positions of three sperms in two successive frames (white spots show the sperms’ end positions and black spots show their starting positions). The displacement vectors of these sperms are labeled as S1, S2, and S3.
