Figure 5.
Counting accuracy of the presented automated semen analysis platform for (a) immotile sperms; (b) motile sperms; and (c) both the motile and immotile sperms is illustrated at various sperm concentrations up to 12.5 × 106/mL. The x-axes are the sperm concentrations that are manually counted using a conventional bright-field microscope. The y-axes are the sperm concentrations that are automatically counted for the same semen samples using lensfree holographic images acquired with the on-chip microscope shown in Figure 1(a). The total counts in (c) are the summed up concentrations of the immotile sperms in (a) and the motile sperms in (b). Correlation coefficients (r) of these characterization results shown in (a), (b), and (c) (0.98, 0.99, and 0.98, respectively) further validate the accuracy of this compact and lightweight holographic lensfree microscope as a semen analysis platform.