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. Author manuscript; available in PMC: 2011 Dec 3.
Published in final edited form as: Neurosci Lett. 2010 Sep 17;486(1):47–50. doi: 10.1016/j.neulet.2010.09.042

Figure 1. Neuromelanin is not cytotoxic to TNFα-stimulated human A172 astroglial cells.

Figure 1

A172 cells were cultured in serum free medium for 48 h in the presence or absence of NM (0.02-15 μg/ml), then co-exposed to human recombinant TNFα (5ng/ml) for an additional 24 h. To assess mitochondrial integrity/cell viability, the MTT assay was performed. Data were normalized as percent control (TNFα alone) within each experiment and presented as the mean + S.E.M. of triplicate measures from 2-3 independent experiments. No significant differences (P < 0.05) among treatments were detected by one-way ANOVA.