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. Author manuscript; available in PMC: 2011 Dec 3.
Published in final edited form as: Neurosci Lett. 2010 Sep 17;486(1):47–50. doi: 10.1016/j.neulet.2010.09.042

Figure 2. Neuromelanin inhibits TNFα-induced CXCL10 protein expression in human A172 astroglial cells.

Figure 2

A172 cells were cultured in serum free medium for 48 h in the presence or absence of NM (0.02-15 μg/ml), then co-exposed to human recombinant TNFα (5ng/ml) for an additional 24 h. Secreted CXCL10 protein in the culture media was then quantitated using a standard dual-antibody solid phase immunoassay, according to the manufacturer's instructions as previously described [12]. Absorbance was read at 450 nm (with wavelength correction set at 650 nm) on a BIOTEK HT spectrophotometer. Data were normalized as percent control (TNFα alone) within each experiment and presented as the mean + S.E.M. of triplicate measures from 3-4 independent experiments. Statistical differences determined by one-way ANOVA with Dunnett's post-hoc comparisons. *P < 0.05 vs TNFα; **P < 0.01 vs TNFα.