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. 2010 Nov 3;11:43. doi: 10.1186/1471-2091-11-43

Figure 3.

Figure 3

VEGF165 binding to PlnDI in solution is pH dependent. PlnDI pre-incubated with VEGF165 in 25 mM HEPES (pH 6.0, 7.0, or 8.0) was immobilized on nitrocellulose. VEGF165 bound to PlnDI was detected with VEGF specific monoclonal antibodies. (A) pH dependent binding of VEGF165 to PlnDI. (B) The requirement of CS and HS chains for VEGF165 binding to PlnDI (pH 7.0). Data are presented as mean specific density values (DV) of triplicate determinations ± SEM. Specific binding was determined by subtracting the background binding of VEGF165 to nitrocellulose from total bound. (*) different from pH 8.0; (**) different from pH 7.0; (φ) different from PlnDI+VEGF165; (θ), different from PlnDI+Hepase+VEGF165. p < 0.05.