Figure 6.

PlnDI stimulates VEGFR-2 (Tyr-951) phosphorylation. Human bone marrow endothelial cells without cell surface HS were incubated with VEGF₁₆₅ or PlnDI for 0, 2, 5, 10 and 20 minutes. Cell lysates were analyzed for VEGFR-2 phosphorylation by Western blot using anti-phospho and total VEGFR-2 tyrosine residue 951 specific antibodies. Time dependant increase in VEGFR-2 phosphorylation induced by: (A) VEGF₁₆₅ and (B) PlnDI. (C) PlnDI induced VEGFR-2 phosphorylation: Requirement for CS and HS chains. Exogenous heparin served as a positive control and GAPDH as loading control. Horizontal bars represent the densitometric scanning of bands outlined in rectangular boxes. Data are presented as the mean density values (DV) from triplicate determinations ± SEM. (*) different from 0 min or media. (δ) different from PlnDI. p < 0.05.