Table 1.
Peptides and Peptide-PNAs Included in Studies
| Description | Sequence | Molecular Weight |
|---|---|---|
| Glu-FITC | EEE-FITC | 776 Da |
| Glu-Ala-FITC | EEEAA-FITC | 919 Da |
| Glu-Lys-FITC | EEEAAKKK-FITC | 1303 Da |
| Glu-Oct6-FITC | EEEAAGRKRKKRT-FITC | 1930 Da |
| Oct6- FITC | GRKRKKRT-FITC | 1400 Da |
| Phe-Oct6-FITC | FFFAAGRKRKKRT-FITC | 1984 Da |
| Asn-Oct6-FITC | NNNAAGRKRKKRT-FITC | 1885 Da |
| Phe-Oct6-dansyl | FFFAAGRKRKKRT-dansyl | 1875 Da |
| Asn-Oct6-dansyl | NNNAAGRKRKKRT-dansyl | 2058 Da |
| Glu-Oct6 | EEEAAGRKRKKRT | 1559 Da |
| Phe-Oct6 | FFFAAGRKRKKRT | 1613 Da |
| Tyr-Oct6 | YYYAAGRKRKKRT | 1661 Da |
| Tyr-Oct6-FITC | YYYAAGRKRKKRT | 2032 Da |
| Description | Sequence | |
| FITC-PNA-EEE | FITC-TATGATCTCCTCCGT-EEE | |
| FITC-TAT-PNA | FITC-GRKKRRQRRRRC-TATGATCTCCTCCGT | |
| Glu-Oct6-PNA-FITC | EEEAAGRKRKKRT-TATGATCTCCTCCGT-K-FITC | |
| Glu-Oct6-13410a | EEEAAGRKRKKRT-TCCCGTAAATCCCTA | |
| 13410a | TCCCGTAAATCCCTA | |
Carboxyfluorescein and carboxydansyl peptides were synthesized by FMoc chemistry at the Molecular Resources Facility, UMDNJ. They were purified by HPLC and sequence verified by the same technique. Carboxydansyl peptides are synthesized using lysyl dansyl moieties. The peptide-PNAs were synthesized by BioSynthesis; they were purified by HPLC and verified by MALDI-TOF. The letters in bold indicate the PNA portions of the molecules. The FITC moiety was coupled to Glu-Oct6-PNA as a lysyl-FITC, due to the requirements of PNA chemistry.