Table 4.
Cell line | mean % nuclear FITC | mean nuclear FITC intensity | mean % cyto. FITC | mean cyto. FITC intensity |
---|---|---|---|---|
DU-145 | 68.04 | 274,597 | 22.48 | 76,943 |
PANC-1 | 72.61 | 322,794 | 21.8 | 68,465 |
Cells were incubated with 1000 nM Glu-Oct6-PNA-FITC for 18 hr, at which time cells were fixed with 4% paraformaldehyde, stained with 5 μM DRAQ5 for nuclear localization, and analyzed on an Amnis ImageStream imaging cytometer.
Four to 5 thousand events were collected and analyzed using IDEAS software (Amnis).