Table 4.

Glu-Oct6 Facilitated Nuclear Colocalization of PNA 13778a

Cell line	mean % nuclear FITC	mean nuclear FITC intensity	mean % cyto. FITC	mean cyto. FITC intensity
DU-145	68.04	274,597	22.48	76,943
PANC-1	72.61	322,794	21.8	68,465

Cells were incubated with 1000 nM Glu-Oct6-PNA-FITC for 18 hr, at which time cells were fixed with 4% paraformaldehyde, stained with 5 μM DRAQ5 for nuclear localization, and analyzed on an Amnis ImageStream imaging cytometer.

Four to 5 thousand events were collected and analyzed using IDEAS software (Amnis).