Figure 5. Induction of metallothionein gene expression during the formation of cross-adaptation response to neurobehavioral toxicity induced by Pb exposure.

(A) Induction of green fluorescence protein in the Pmtl-1::GFP and Pmtl-2::GFP transgenic nematodes. (B) mtl-1 and mtl-2 transcription assay. Relative expression ratio (between mtl-1 or mtl-2 gene and ubq-1 reference gene) in treatments are normalized to the control. L2-stage larvae animals were heat stressed for 1 h at 36°C. The exposed metal concentrations were 50 and 100 µM. Bars represent means ± S.D.