Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Nov 18;5(11):e14033. doi: 10.1371/journal.pone.0014033

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A, BrdU incorporation assay indicated that cell proliferation was significantly induced in POb cells when co-cultured with FOb cells. B, osteoblast differentiation of FOb and POb co-cultures was investigated by Alizarin red staining and its quantification. Osteogenic differentiation of POb cells was significantly enhanced when co-cultured with FOb cells, while co-cultures of FOb or POb cells with POb cells showed negative effects. Cells cultured alone were used as controls. C, BrdU incorporation of FOb and POb cells cultured with FOb- and POb- derived conditioned media (FCM & PCM, serum free) revealed that FMC significantly increased cell proliferation on both, FOb and POb cells at 24 hours. Serum free medium was used as control. D, osteogenic differentiation of FOb and POb cells in presence of differentiation –FCM (dFMC) and differentiation-PCM (dPMC) supplemented with 3% FCS, was measured and quantified by Alizarin Red staining. A greater osteogenic differentiation was observed in both FOb and POb cells only when incubated with dFCM, but not with dPCM. Osteogenic differentiation medium supplemented with 3% FCS was used as control.