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. 2010 Nov 18;5(11):e14033. doi: 10.1371/journal.pone.0014033

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Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Endogenous levels of FGF-2 were analyzed on FOb and POb cells by immunoblotting using antiFGF-2 antibody. Higher levels of high molecular weight (HMW) and low molecular weight (LMW) FGF-2 forms were observed in FOb compared to POb at all stages analyzed. The total cellular FGF-2 proteins were quantified by densitometry (lower panel), α-tubulin was used as loading control. Histogram represents the densitometric analysis of electrophoresis bands, the relative intensities of bands were normalized to their respective loading control and set as 100% The results are presented as the mean ± SD of three independent experiments. The asterisks represent P value <0.001.