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. 2010 Nov 18;5(11):e14058. doi: 10.1371/journal.pone.0014058

Figure 2. The domain structures and the developmental expression pattern of the two EcR isoforms in S. exigua.

Figure 2

(A) The domain structures of SeEcR-A and SeEcR-B1 and the common fragment used to generate the dsRNAs. The number of amino acids of each domain is indicated. (B) The developmental expression profiles of SeEcR-A and SeEcR-B1 mRNA. Total RNAs were prepared from individuals of S. exigua collected at all developmental stages from the first day of eggs to the last day of adults. The levels of the mRNA were detected using qRT-PCR. The mRNA level of β-Acitn was used as a control to normalize for loading. The data represent the mean values ± SE of three replicates. The age of the insects is indicated in: E1, the first day of eggs; I1, the first day of the first instar larvae; pre, prepupal period; P1, the first day of pupae; A1, the first day of adults.