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. 2010 Nov 18;5(11):e14058. doi: 10.1371/journal.pone.0014058

Table 2. Primers used in this study.

Information about the primers
For dsRNA
Name of primers Nucleotide sequence (5′–3′)
dsEcR-F* GGATCCTAATACGACTCACTATAGG GTGTCGGTTGAAGAAATGTCT
dsEcR-R* GGATCCTAATACGACTCACTATAGG CGCAACATCATCACCTCACT
dsEcR-SF GTGTCGGTTGAAGAAATGTCT
dsEcR-SR CGCAACATCATCACCTCACT
dsGFP-F* GGATCCTAATACGACTCACTATAGG AAGGGCGAGGAGCTGTTCACCG
dsGFP-R* GGATCCTAATACGACTCACTATAGG CAGCAGGACCATGTGATCGCGC
dsGFP-SF AAGGGCGAGGAGCTGTTCACCG
dsGFP-SR CAGCAGGACCATGTGATCGCGC
For SeEcR cloning
Name of primers Degenerate primers (5′–3′)
SeEcR-F CCNCGGCARCARGAGGAGC
SeEcR-R TCCTCBTCNGANGGCTG
Nested PCR primers (5′–3′)
5-SeEcR-1 GAATCGTGGCACCACCTCGTGAAT
5-SeEcR-2 GAGGCGGTGGATCACACTGCAT
3-SeEcR-1 GTGTCGGTTGAAGAAATGTCT
3-SeEcR-2 ATGCAGTGTGATCCACCGCCTC
For quantitative real-time RT-PCR
Gene Forward (5′–3′) Reverse (5′–3′)
SeEcRcom GTGAGGTGATGATGTTGCGAGTA AGAAAATGACGATGGCAGTGAG
SeTre-1 GGTTGGGACTTCTCAACGCGC GACAGCATCCCGGTGATTCCC
SeTre-2 GGCAGGCGTCGGGATTACTTC GGCCAGGCATTCGGATAGTCC
SeG6PI ATCAGTGGACAGTGGAAAGGGTA TTGAGGTGGTTGGCGTAAGG
SeUAP CCTGATGGCAACGGAGGACTG CAACTTTCGCCGCACAGTCAG
SeCHSA GGCTCGGGTCCTCATAACGTC GTACCTGGTCCATCGTTGGCG
SeCHSB GCTCGGTTCTGCGGTTGTGTC CCATAGCCACGTCACACGCTC

* The sequence in bond at the 5′ end of the primer is T7 promoter sequence.