Table 2. Primers used in this study.
| Information about the primers | |||
| For dsRNA | |||
| Name of primers | Nucleotide sequence (5′–3′) | ||
| dsEcR-F* | GGATCCTAATACGACTCACTATAGG GTGTCGGTTGAAGAAATGTCT | ||
| dsEcR-R* | GGATCCTAATACGACTCACTATAGG CGCAACATCATCACCTCACT | ||
| dsEcR-SF | GTGTCGGTTGAAGAAATGTCT | ||
| dsEcR-SR | CGCAACATCATCACCTCACT | ||
| dsGFP-F* | GGATCCTAATACGACTCACTATAGG AAGGGCGAGGAGCTGTTCACCG | ||
| dsGFP-R* | GGATCCTAATACGACTCACTATAGG CAGCAGGACCATGTGATCGCGC | ||
| dsGFP-SF | AAGGGCGAGGAGCTGTTCACCG | ||
| dsGFP-SR | CAGCAGGACCATGTGATCGCGC | ||
| For SeEcR cloning | |||
| Name of primers | Degenerate primers (5′–3′) | ||
| SeEcR-F | CCNCGGCARCARGAGGAGC | ||
| SeEcR-R | TCCTCBTCNGANGGCTG | ||
| Nested PCR primers (5′–3′) | |||
| 5-SeEcR-1 | GAATCGTGGCACCACCTCGTGAAT | ||
| 5-SeEcR-2 | GAGGCGGTGGATCACACTGCAT | ||
| 3-SeEcR-1 | GTGTCGGTTGAAGAAATGTCT | ||
| 3-SeEcR-2 | ATGCAGTGTGATCCACCGCCTC | ||
| For quantitative real-time RT-PCR | |||
| Gene | Forward (5′–3′) | Reverse (5′–3′) | |
| SeEcRcom | GTGAGGTGATGATGTTGCGAGTA | AGAAAATGACGATGGCAGTGAG | |
| SeTre-1 | GGTTGGGACTTCTCAACGCGC | GACAGCATCCCGGTGATTCCC | |
| SeTre-2 | GGCAGGCGTCGGGATTACTTC | GGCCAGGCATTCGGATAGTCC | |
| SeG6PI | ATCAGTGGACAGTGGAAAGGGTA | TTGAGGTGGTTGGCGTAAGG | |
| SeUAP | CCTGATGGCAACGGAGGACTG | CAACTTTCGCCGCACAGTCAG | |
| SeCHSA | GGCTCGGGTCCTCATAACGTC | GTACCTGGTCCATCGTTGGCG | |
| SeCHSB | GCTCGGTTCTGCGGTTGTGTC | CCATAGCCACGTCACACGCTC | |
* The sequence in bond at the 5′ end of the primer is T7 promoter sequence.