Table 1. Summary of synthesized peptides used; Kd taken from [12].
Peptide | Sequence | Kd×10−13 [M] |
CKII(290–312) | CLKKFNARRKLKGAILTTMLATRN | 3 |
CKII(292–312) | CKFNARRKLKGAILTTMLATRN | 5 |
CKII(293–312) | CFNARRKLKGAILTTMLATRN | 17 |
CKII(294–312) | CNARRKLKGAILTTMLATRN | 570 |
CKII(290–312)* | LKKFNARRKLKGAILTTMLATRN | 3 |
*: high affinity peptide used for unspecific background determination as described in supporting Material S1. This peptide cannot bind to the crosslinker (Assay CaM/CKII) due to the absence of an N-terminal Cys residue.