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. 2010 Nov 18;6(11):e1001202. doi: 10.1371/journal.ppat.1001202

Figure 9. Mutation of lysine 383 in PopP2 abolishes RRS1-R-mediated immunity.

Figure 9

A: PopP2, PopP2-C321A and PopP2-K383R are detected in total extracts and supernatants of ΔpopP2 and ΔpopP2/Δhrcv bacterial cultures. B: Nd-1 plants expressing the RRS1-R gene were root-inoculated with the ΔpopP2 strain-expressing wild-type PopP2 (dark grey bars), PopP2-C321A (black bars), PopP2-K268R, PopP2-K285R, PopP2-K335R (all represented by clear grey bars) or PopP2-K383R (empty bars). Bacterial growth was measured 4, 5 and 7 days after inoculation. Means of colony-forming units per gram of fresh weight (cfu/gfw) were calculated from triplicates of three plants. For each time point, error bars represent standard deviation from three triplicates. Shown values are representative of two additional replicates.