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. 2010 Nov 18;6(11):e1001202. doi: 10.1371/journal.ppat.1001202

Table 1. FRET-FLIM measurements showing that RRS1-R and RRS1-S proteins interact with PopP2 in the nucleus of plant cells.

Transient assay in Donor Acceptor τ. sem(a) Δb) N(c) E(d) p-value
N. benthamiana PopP2-CFP - 2.386 0.030 - 130 - -
N. benthamiana PopP2-CFP RRS1-R-YFPv 1.979 0.024 407 110 19 6.4E-21
N. benthamiana PopP2-CFP YFPv 2.361 0.027 - 25 - 0.720
N. benthamiana PopP2-CFP RRS1-R 2.391 0.035 - 25 - 0.943
N. benthamiana PopP2-C321A-CFP - 2.375 0.019 - 30 - -
N. benthamiana PopP2-C321A-CFP RRS1-R-YFPv 2.021 0.031 354 30 15 8.3E−14
A. thaliana PopP2-CFP - 2.297 0.035 - 10 - -
A. thaliana PopP2-CFP RRS1-R-YFPv 1.872 0.031 425 8 18 1.5E−07
N. benthamiana PopP2-CFP RRS1-S-YFPv 1.889 0.031 497 81 21 1.8E−22
N. benthamiana PopP2-K383R-CFP - 2.362 0.027 - 30 - -
N. benthamiana PopP2-K383R-CFP RRS1-R-YFPv 2.064 0.023 298 46 12.6 3.4E−12

*Mean lifetime, τ, in ns. (a) for each nucleus, average fluorescence decay profiles were plotted and fitted with exponential function using a non linear square estimation procedure and the mean lifetime was calculated according to τ = Σ αiτi 2/Σ αiτi with I(t)  = Σ αi e−t/τi , standard error of the mean, (b) Δt = τD - τDA (in ps), (c) total number of measured nuclei , and (d) % FRET efficiency: E = 1 – (τDA/τD). p-value of the difference between the donor lifetimes in the presence and in the absence of acceptor (Student's t test).