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Early time course of T cell division and expansion in response to anti-CD3/CD28 beads and soluble anti-CD3. CFSE-labeled CD4 (A) and CD8 (C) cells began dividing 40-60 hours after exposure to beads (solid lines) or anti-CD3 (dashed lines), but divided minimally in the absence of stimulation (2× solid lines). The number of stimulated and control CD4 (B) and CD8 (D) T cells remained comparable until about 60 hours after stimulation when measureable cell expansion began.
