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. 2010 Oct 26;8:104. doi: 10.1186/1479-5876-8-104

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Copyright ©2010 Li and Kurlander; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Early time course for expansion of bead-expanded T cells after restimulation 14 days later with anti-CD3/CD28 beads or anti-CD3 plus irradiated MMCs. Restimulated CD4 (A) and CD8 (C) both divided more extensively in response to anti-CD3 (dashed lines) than anti-CD3/CD 28 (solid lines), but this difference was more pronounced for CD8 cells. CD4 T cell expansion (C) was similar in response to either stimulus, but CD8 T cells (D) expanded substantially more rapidly than anti-CD3/CD28 bead-treated cells.