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Comparison of changes in forward scatter measured by flow cytometry (a measure of cell size) in response to anti-CD3/CD28 beads and anti-CD3. Primary CD4 (A) and CD8 cells (B) showed similar changes in forward scatter after exposure to either stimulus, but CD4 (C) and CD8 (D) cells restimulated on day 30 increased in size more slowly and remained large for longer after restimulation with beads. This difference in size was noted even when the enlarged cells were expanding poorly.
