Table 4.
Correlation between microarray ad qRT-PCR data
| Target Gene | PIPA ORF | 8/21 E array | p-value | 8/21 E qPCR | stdev |
| MDH1 | PIPA02244 | -1.61 | 4.90E-03 | -2.03 | ± 0.212 |
| FUM1 | PIPA02844 | -2.07 | 2.23E-02 | -1.42 | ± 0.121 |
| YDL124W | PIPA01263 | 2.58 | 3.00E-04 | 1.52 | ± 0.139 |
| RKI1 | PIPA02895 | 2.44 | 2.50E-03 | 1.93 | ± 0.091 |
| CDC19 | PIPA00751 | 5.24 | 8.24E-10 | 3.71 | ± 0.189 |
| TDH3 | PIPA02510 | 4.34 | 5.44E-06 | 2.03 | ± 0.151 |
| Fab Hc | - | 1.65 | 8.80E-02 | 1.68 | ± 0.064 |
| Fab Lc | - | 2.07 | 8.30E-05 | 1.80 | ± 0.019 |
| E/C 8 array | p-value | E/C 8 qPCR | stdev | ||
| NCE103 | PIPA03864 | 2.21 | 2.30E-02 | 1.71 | ± 0.087 |
| PFK3 | PIPA09969 | 2.53 | 1.20E-02 | 3.21 | ± 0.128 |
| ERG25 | PIPA00945 | 2.79 | 3.00E-03 | 2.21 | ± 0.093 |
| AQR1 | PIPA04502 | 3.18 | 3.90E-02 | 4.12 | ± 0.188 |
| YCT1 | PIPA00376 | -9.19 | 3.90E-04 | -5.03 | ± 0.322 |
| FLR1 | PIPA02458 | -2.22 | 4.10E-02 | -1.78 | ± 0.161 |
| Fab Hc | - | 2.96 | 1.75E-01 | 10.31 | ± 0.397 |
Quantitative real-time PCR results compared with microarray data. Standard deviations (stdev) derive from triplicate measurements; all numbers reflect relative gene expression to the reference gene actin (ACT1). PIPA ORF = Pichia pastoris Open Reading Frame (http://www.pichiagenome.org); 8/21 E = pairwise comparison of hypoxic (8) and normoxic (21) conditions in the expressing strain (E); E/C 8 = pairwise comparison of expressing and control (C) strain under hypoxic conditions