Table 4.
U937 cells' functional status after the freezing - thawing cycle.
| Parameters measured (au) | Cell status and subgroups | ||||
|---|---|---|---|---|---|
| Live cells (62%) | Late apoptosis (18%) | Early apoptotic cells | |||
| Total (19%) | MMP (+) (5%) | MMP (-) (14%) | |||
| TMRM mean FI | 1476 ± 331 | Negative | 242.7 ± 155.6 | 429 ± 131 | Negative |
| TMRM FI SD | 652 ± 249 | Negative | 97.2 ± 11.2 | 141 ± 47 | Negative |
| FDA slop | 36.1 ± 11.36 | Negative | 9.6 ± 11.2 | 11 ± 7.1 | 9.05 ± 12.2 |
U937 cells underwent a freezing - thawing cycle as described in Materials and Methods. Overnight after thawing the multi-parametric analysis was performed and four parameters were elicited for each single cell. The cell subgroups were defined as described in Multi-parametric analysis following the freezing - thawing cycle in the i3C section. Values are average ± SD in individual cells' subgroups. The percentage of each subgroup from the total population in a representative experiment is shown in brackets.