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SDS-polyacrylamide gel of overexpressed esterase EstMY in E. coli. Lane 1: molecular weight protein marker (Tiangen, Cat. No: MP203); lane 2, E. coli/pET28a: total protein extract, as negative control; lane 3: induced culture of E. coli/pET28a, as negative control; lane 4: total protein extract E. coli/pEstMY-His; lane 5, total protein extract, induced culture of E. coli/pEstMY-His; lane 6: purified EstMY (38 kDa).
