Figure 5.
Substrate specificity of overexpressed and purified esterase EstMY. Specific activity of hydrolysis of different p-nitrophenyl esters. p-NP acetate (C2), p-NP butyrate (C4), p-NP hexanoate (C6), p-NP caprylate (C8), p-NP decanoate (C10), p-NP laurate (C12), p-NP myristate (C14), and p-NP palmitate (C16). Relative activity was shown as the percentage of the activity of the activity towards 4-nitrophenyl caprylate. All measurements were performed in triplicate.