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Effect of pH on the purified esterae EstMY. Relative activity of p-NP-caprylate hydrolysis was performed in various pH buffers at 35°C (pH 5.0-7.5, 50 mM phosphate buffer; pH 8.0-10.5, 50 mM Tris-HCl buffer). The activity at pH 8.5 was set as 100% (4,615 U/ml). All measurements were performed in triplicate.
