Table 2.
Primers used in the study
| Primer | Sequence 5'-3' | Description |
|---|---|---|
| HTFP061 | GTACAACGACACCTAGAC | Sequencing primer for pCC1FOS™ |
| HTRP062 | CAGGAAACAGCCTAGGAA | Sequencing primer for pCC1FOS™ |
| M13 primer RV | CAGGAAACAGCTATGAC | Sequencing primer for pUC18 |
| M13 primer M2 | AGCTGTTCACCGAAGTGCTG | Sequencing primer for pUC18 |
| EstMY -W1F | CGCCCCTTTCGACCAGCAACG | Genomic walking primer for estMY gene |
| EstMY -W2F | CTACGCCGACCTCACCGGCCT | Genomic walking primer for estMY gene |
| EstMY -W1R | GAGGGGTGTGCGGGGATGCG | Genomic walking primer for estMY gene |
| EstMY -W2R | GACGTAGCCGCCGCCGTGAAG | Genomic walking primer for estMY gene |
| EstMY -F | GGCATATGGCCGCGCCCGTTCCGCCCATCAG NdeI | Forward primer for estMY gene |
| EstMY -R | GGAAGCTTCTACGCTGCCGCCCTAGCGCCGATGHindIII | Reverse primer for estMY gene |
The NdeI and HindIII sites are underlined. The start codon is in bold.