Table 4.

Effect of detergents and enzyme inhibitors on esterase activity

Compounds	Concentration	Relative activity (%)
Control	0	100.0 ± 2.1
β-mercaptoethanol	3 mM	101.7 ± 2.6
DTT	3 mM	106.9 ± 4.9
CTAB	3 mM	129.7 ± 2.2
DEPC	3 mM	38.6 ± 2.7
PMSF	3 mM	101.3 ± 4.1
SDS	3 mM	12.3 ± 2.9
Triton X-100	0.5%	129.6 ± 4.6
Tween 80	0.5%	138.4 ± 2.1
Tween 20	0.5%	156.7 ± 3.3

Activity without detergents and enzyme inhibitors was set as 100% (4,970 U/ml). All measurements were repeated three times.