Figure 2.

Effects of Taurolidine (TRD) 250 μM on viability, apoptosis and necrosis in different malignant cell lines. HT29, Chang Liver, HT1080, AsPC-1 and BxPC-3 cells were incubated with TRD 250 μM and with Povidon 5% (control) for 24 h. The percentages of viable (a), apoptotic (b) and necrotic cells (c) were determined by FACS-analysis for Annexin V-FITC and Propidiumiodide. Values are means ± SEM of 9 (HT29), 10 (Chang Liver, BxPC-3) or 11 (HT1080, AsPC-1) independent experiments with 4-5 consecutive passages. Asterisk symbols on columns indicate differences between control and TRD treatment. *** p ≤ 0.001, ** p ≤ 0.01, * p ≤ 0.05 (one-way ANOVA followed by Tukey's post-hoc test)