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Western blot analysis of selected genes targeted by Taurolidine treatment in five malignant cell lines. HT29, Chang Liver, HT1080, AsPC-1 and BxPC-3 cells were incubated with Taurolidine (TRD) 250 μM and with Povidon 5% (control) for 8 h. Western blot analysis was performed for validation of altered gene expression observed during microarray experiments for ATF3 (a) and PPP1R15A (b). GAPDH served as loading control.
