Figure 2. Cryo-EM of S-domain intermediate with the helical extension on helix P9 (P9ext, green).
(A) P9ext (blue circles) has the same folding behavior as the wild-type S-domain (black line). (B) Cryo-EM reconstruction of the P9ext intermediate. The final 3D map was generated using 60 class averages from ~19,800 particles.