Table 1.
Oligonucleotide primers used for the construction of infectious cDNA clone of the avian HEV-VA strain recovered from a clinically healthy chicken.
| Primer ID | Primer sequences (5′ to 3′)a |
|---|---|
| Fragment 1 (f1) | |
| MluIT7F1 | cacgcgttaatacgactcactataGCATGACCCCATGCCAGGGT (Mlu I) |
| AflII1690R1 | GCCGGTGATACGCTGCTGAC |
| Fragment 2 (f2) | |
| AflII1542F2 | TGACAGCACGGAGGATTTGA |
| EcoRV3826R2 | TAATAATAACCCGGGGGCAG |
| Fragment 3 (f3) | |
| EcoRV3727F3 | CATGGTAAAGTGGGACAGGG |
| RsrII5349R3 | ATGAGCATGCCAGACGTAGC |
| Fragment 4 (f4) | |
| RsrII5279F4 | GACCATACCACACGAGCGTT |
| T18BamHIR4 | gcggatccttttttttttttttttttACTATGCCCGAGATG (Bam HI) |
a
Lower-case letters indicate non-viral sequences. The T7 core promoter sequence in primer MluIT7F1 is underlined. The restriction enzyme sites introduced by PCR are shown in italics and specified in parentheses. Fragments 1–4 correspond to the fragment numbers in Fig. 1.