. Author manuscript; available in PMC: 2012 Jan 27.

Published in final edited form as: Vet Microbiol. 2010 Jul 22;147(3-4):310–319. doi: 10.1016/j.vetmic.2010.07.016

Table 2.

Detection of avian HEV RNA in specific-pathogen-free chickens experimentally inoculated with capped RNA transcripts from infectious cDNA clones of avian HEV-VA strain (pT7-aHEV-VA) recovered from a healthy chicken, and avian HEV-prototype strain (pT7-aHEV-5) recovered from a chicken with HS syndrome.

Group	Samples	No. of positive samples/total no. tested at indicated wpi						Overall no. of infected chickens in each group^b
Group	Samples	0	1	2	3^a	4	5^a	Overall no. of infected chickens in each group^b
Avian HEV-VA (pT7-aHEV-VA)	Feces	0/10	6/10	4/10	2/10	1/5	1/5
	Serum	0/10	1/10	1/10	4/10	0/5	0/5	10/10
	Bile	-	-	-	4/5	-	1/5
Avian HEV-prototype (pT7-aHEV-5)	Feces	0/9^c	2/9	4/9	1/9	1/4	0/4
	Serum	0/9^c	2/9	0/9	1/9	0/4	0/4	9/9
	Bile	-	-	-	2/5	-	0/4

Five chickens were necropsied at 3 wpi and the remaining chickens were necropsied at 5 wpi. Samples from all chickens in the control group remained negative throughout the experiment and were not included in the table.

No. of chickens which had detectable avian HEV RNAs by RT-PCR in feces, sera, or bile at certain points during experiment.

One chicken died 1 day after inoculation.