Table 1.
Primer sequences used and results of confirmative quantitative RT–PCR analysis of 10 genes that were observed to be up-regulated by L-methionine
| Gene symbol | Description of gene | LNCaP |
MCF-7 |
Primer sequences |
|||
|---|---|---|---|---|---|---|---|
| Fold change | P value | Fold change | P value | Forward | Reverse | ||
| LAMA3 | Laminin, alpha 3 | 4.58 | 7.62E-04 | 4.96 | 4.19E-03 | TCCCTATTTGCCAAGCCT | ACCGCTGTCCTGTAACT |
| AKR1C2 | Aldo–keto reductase family 1, member C2 | 13.28 | 1.21E-03 | 3.57 | 1.45E-03 | The primers used were from the Applied Biosystems Hs00912742_m1 protocol | |
| NQO1 | NAD(P)H dehydrogenase, quinone 1 | 3.53 | 8.09E-06 | 2.44 | 9.16E-04 | CAACCACGAGCCCAG | AGTGAGCCAGTACGATCAG |
| RBM4B | RNA binding motif protein 4B | 2.57 | 1.65E-02 | 2.50 | 9.97E-03 | GGTATGAACGGGAGCAGTAT | GCGGAGCAAGTTCTCAT |
| AFF3 | AF4/FMR2 family, member 3 | 2.47 | 1.20E-02 | 5.64 | 1.99E-04 | CAAGTTCAGCATCCCCAA | GTGGAAGCCAGGTCATCT |
| SH3BGRL | SH3 domain binding glutamic acid-rich protein-like | 2.46 | 1.14E-03 | 2.26 | 4.23E-03 | TGTTCCCAGGATGGTGAT | CTTCTAGGAAACCAAGCACA |
| GPX8 | Glutathione peroxidase 8 | 2.00 | 4.00E-03 | 4.91 | 1.64E-03 | CTCTGGAAAAGTATAAAGGCAAAG | TTGTGCAGTTCCTTCAGC |
| VAMP5 | Vesicle-associated membrane protein 5 (myobrevin) | 3.03 | 1.56E-03 | 1.78 | 8.84E-02 | CCTCAGAGCAGTGACAGCAG | CCATTTGGCTTCTCCTTCAG |
| C8orf4 | Chromosome 8 open reading frame 4 | 2.06 | 2.34E-03 | 3.44 | 7.84E-03 | GGAAGATCCCCACATCGAT | TCAAAGATGTTGCCCACG |
| ATF3 | Activating transcription factor 3 | 2.05 | 1.57E-03 | 3.08 | 1.48E-03 | CATCACAAAAGCCGAGGT | AGCTTCTCCGACTCTTTCTG |