Figure 7. Increased COX 2 expression in Bmal1-KO endothelial cells.
Mouse aortic endothelial and smooth muscle cells were isolated from Bmal1-KO and WT mice (see methods). Isolated cells were cultured and protein lysates were isolated and separated by SDS-PAGE electrophoresis. (A) Expression levels of COX-2 but not COX-1 were significantly increased in endothelial cells from Bmal1-KO mice. (B) COX-2 and COX -1were not significantly different in isolated smooth muscle cells from Bmal1-KO and WT control mice. Changes were quantified by densitometry (*p<0.05 versus WT; N=6)