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Time-dependence of RTV-induced ΔΨm depolarization in HL-1 myocytes. Ratios of JC-1 fluorescence (FL1/FL2) normalized by control fluorescence ratio are plotted for RTV (15 μM; n = 5), APV (15 μM; n = 4), and a time control (Ctrl; n = 3). Treatment with DNP (0.3 mM, 20 min) served as positive control. Increased FL1/FL2 corresponds to depolarization of ΔΨm.
