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. 2010 Sep 27;285(48):37133–37137. doi: 10.1074/jbc.C110.169763

FIGURE 3.

FIGURE 3.

NAADP is vasoactive. A, left, effect of NAADP-AM (500 nm) on membrane potential in a control cell (solid line) and a cell pretreated for 15 min with 1 μm apamin and 50 nm charybdotoxin (dashed trace). Right, effect of bafilomycin A1 pretreatment (BAF, 1 μm, 1 h). Data are representative of 12–19 cells. B, left, effect of NAADP-AM (500 nm) on NO production in a control cell (solid line) and a cell pretreated for 15 min with 10 μm l-NAME (dashed trace). Right, effect of bafilomycin A1 pretreatment (BAF, 1 μm, 1 h). Data are representative of 18–34 cells. C–E, the effect of KCl (40 mm), acetylcholine (ACh, 10 μm), and NAADP-AM (500 nm) on contractility of aortic rings with the endothelium intact (C and D) or following its removal (E). In D, l-NAME (100 μm) was added prior to NAADP-AM (500 nm). Washout of the various agents is marked. Data are representative of 5–6 preparations. F, effect of intravenous injection of NAADP-AM (300 μl, 1 μm) on pulsatile arterial pressure (PAP, top) and mean arterial pressure (MAP, bottom) in anesthetized rats in the absence (left traces) or presence of (right traces) of l-NAME (5 mg/kg). Data are representative of 5–6 animals.