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Protein constructs and DNA oligos used in this study. A, sequence of the C-terminal domain of NPM1. The region differentiating NPM1-C70 from NPM1-C53 (whose three-dimensional structure is shown in the inset) is underlined. Mutated lysines are shown in bold. The PSIPRED prediction is also shown (C = coil, H = α-helix, E = β-strand). B, oligos used in this study are shown both in sequence and in putative structure.
