FIGURE 2.

SPR sensorgrams of the interaction between G10-loop and NPM1 constructs. The oligo, biotinylated at its 5′ end, was immobilized on a SA (streptavidin) sensor chip. A, experimental curves (dashed lines with circles) represent different concentrations of NPM1-C70 used as the analyte and were fitted according to a single exponential binding model with 1:1 stoichiometry (continuous lines). B, experimental curves represent different concentrations of NPM1-C53. Both on and off rates were too rapid to be fitted. C, Scatchard plot used to determine the dissociation constant with NPM1-C53.