TABLE 3.

Effect of single alanine mutations on NPM1-C70 binding to G10-loop and c-MYC oligos

	G10-loop			c-MYC
	KD	kon	koff	KD	kon	koff
	μm	m−1s−1	s−1	μm	m−1s−1	s−1
NPM1-C70	7.2 ± 0.2	2.8 ± 0.1 × 102	1.98 ± 0.03 × 10−3	1.9 ± 0.1	7.4 ± 0.3 × 103	1.37 ± 0.03 × 10−2
K229A	14.7 ± 0.8	1.6 ± 0.2 × 102	2.30 ± 0.04 × 10−3	10.3 ± 0.9	7.3 ± 0.4 × 102	7.5 ± 0.3 × 10−3
K230A	10.9 ± 0.3	1.50 ± 0.02 × 102	1.60 ± 0.02 × 10−3	15 ± 1	2.8 ± 0.1 × 102	4.3 ± 0.1 × 10−3
K233A	7.5 ± 0.5	4.5 ± 0.2 × 102	3.4 ± 0.1 × 10−3	17.2 ± 0.6	3.0 ± 0.1 × 102	5.2 ± 0.1 × 10−3
K236A	8.1 ± 0.2	3.3 ± 0.1 × 102	2.70 ± 0.02 × 10−3	10 ± 1	2.8 ± 0.2 × 102	2.7 ± 0.2 × 10−2
K239A	19.8 ± 0.5	1.04 ± 0.02 × 102	2.05 ± 0.02 × 10−3	6.8 ± 0.9	8.7 ± 0.7 × 102	5.9 ± 0.3 × 10−3
K229A/K230A	135 ± 8			78 ± 7