TABLE 4.
Urea-induced denaturation of NPM1-C70 and NPM1-C53 in the presence of increasing salt amounts
Urea1/2 and mD-N were obtained by fitting experimental data. ΔG values are calculated as the product of Urea1/2 and mD-N values.
| mD-N NPM1-C70 | mD-N NPM1-C53 | Urea1/2 NPM1-C70 | Urea1/2 NPM1-C53 | ΔG NPM1-C70 | ΔG NPM1-C53 | |
|---|---|---|---|---|---|---|
| No salt | 0.93 ± 0.16 | 0.787 ± 0.07 | 3.76 ± 0.15 | 2.55 ± 0.07 | 3.49 | 2.02 |
| 0.25 m NaCl | 0.93 ± 0.12 | 0.864 ± 0.08 | 4.51 ± 0.11 | 3.56 ± 0.09 | 4.19 | 3.06 |
| 0.50 m NaCl | 0.82 ± 0.11 | 0.843 ± 0.08 | 4.55 ± 0.14 | 3.91 ± 0.08 | 3.77 | 3.29 |
| 1.00 m NaCl | 0.79 ± 0.11 | 0.747 ± 0.09 | 6.20 ± 0.29 | 5.06 ± 0.17 | 4.9 | 3.79 |