FIGURE 4.

LATS and CK1 promote TAZ degradation via the C-terminal phosphodegron. A, Ser-311 and Ser-314 are important for TAZ degradation. MCF10A cells stably expressing TAZ^WT, TAZ^S311A, TAZ^S3114A, and TAZ^DG were chased with CHX treatment as indicted. TAZ stability was determined by WB. Relative TAZ levels were quantified by the ratio of TAZ to actin. B, Ser-311 and Ser-314 are required for TAZ destabilization by LATS and CK1ϵ. TAZ WT and different mutants were co-transfected with or without LATS and/or CK1ϵ as indicated. TAZ expression level was determined by WB. The co-transfected GFP and endogenous actin were included as controls. C, CK1 inhibitor increases endogenous TAZ protein levels. HeLa cells were treated with or without IC261 at different doses and time points. WB was performed to determine TAZ expression levels along the actin control. D, C-terminal phosphodegron is required for TAZ stabilization by CK1 inhibitors. The indicated plasmids were transfected into 293T cells. Treatments with or without the IC261 were indicated. TAZ expression level was determined by WB.